Composite

Part:BBa_K4634004:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-03)


Hypoxia-Induced Promoter Experiment: Control Group


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5242
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5242
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5242
    Illegal BglII site found at 5130
    Illegal XhoI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5242
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5242
    Illegal XbaI site found at 5257
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 303
    Illegal NgoMIV site found at 3350
    Illegal NgoMIV site found at 3510
    Illegal NgoMIV site found at 5098
    Illegal AgeI site found at 5951
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2429


Design Notes

NA


Source

The whole composite part is synthesized by ourselves. For the source of each basic parts, please click on each part to query.

References